For a non-technical introduction to the topic, see Introduction to genetics. This article needs additional citations for verification. Please help improve this article by adding citations human molecular genetics book pdf reliable sources.
Unsourced material may be challenged and removed. Molecular genetics is the field of biology that studies the structure and function of genes at a molecular level and thus employs methods of both molecular biology and genetics. The study of chromosomes and gene expression of an organism can give insight into heredity, genetic variation, and mutations. This is useful in the study of developmental biology and in understanding and treating genetic diseases.
Gene amplification is a procedure in which a certain gene or DNA sequence is replicated many times in a process called DNA replication. DNA nucleotides, template DNA, primers and Taq polymerase. DNA nucleotides make up the DNA template strand for the specific sequence being amplified, primers are short strands of complementary nucleotides where DNA replication starts, and Taq polymerase is a heat stable enzyme that jump-starts the production of new DNA at the high temperatures needed for reaction.
Cloning is the process of creating many identical copies of a sequence of DNA. The target DNA sequence is inserted into a cloning vector. Because this vector originates from a self-replicating virus, plasmid, or higher organism cell when the appropriate size DNA is inserted the “target and vector DNA fragments are then ligated” and create a recombinant DNA molecule. Transformation is the DNA uptake mechanism possessed by bacteria.